ˈ ̷ ̷ ˌ ̷ ̷ ¦ ̷ ̷ - noun
: an in vitro method for quantifying an antigen or antibody concentration in which the test material is immobilized on a surface and exposed either to a complex of an enzyme linked to an antibody specific for the antigen or an enzyme linked to an anti-immunoglobulin specific for the antibody followed by reaction of the enzyme with a substrate to yield a colored product corresponding to the concentration of the test material — called also ELISA