[blot] n [ME] (14c) 1: a soiling or disfiguring mark: spot
2: a mark of reproach: moral flaw
3: a usu. nitrocellulose sheet that contains spots of immobilized macromolecules (as of DNA, RNA, or protein) or their fragments and is used to identify specific components of the spots by applying a molecular probe (as a complementary nucleic acid or a radioactively labeled antibody)
[2]blot vb blot.ted ; blot.ting vt (15c) 1: to spot, stain, or spatter with a discoloring substance
2. obs: mar; esp: to stain with infamy 3 a: to dry (as writing) with an absorbing agent b: to remove with absorbing material "blotting up spilled water" ~ vi 1: to make a blot
2: to become marked with a blot [3]blot n [origin unknown] (1595) 1: a lone backgammon man exposed to capture
2. archaic: a weak or exposed point