KOHLER, GEORGES J.F

born April 17, 1946, Munich, Ger. died March 1, 1995, Freiburg im Breisgau Artificial production of monoclonal antibodies The technique involves fusing in full Georges Jean Franz Khler German immunologist who in 1984, with Csar Milstein and Niels K. Jerne, received the Nobel Prize for Physiology or Medicine for his work in developing a technique for producing monoclonal antibodiespure, uniform, and highly sensitive protein molecules used in diagnosing and combating a number of diseases (see illustration). Khler obtained his doctoral degree in biology (1974) from the University of Freiburg in West Germany. From 1974 to 1976 he worked with Milstein at the Medical Research Council Laboratory of Molecular Biology in Cambridge, England. Together, in 1975, they discovered the technique for which they are known. In the body's immune system, cells called lymphocytes secrete various types of antibodies, whose function is to attach themselves to antigens (foreign substances) that have entered the body. The immune system maintains a vast variety of antibodies, with each type able to attach itself to a matching site on the surface of a particular type of antigen (e.g., a particular species or strain of bacteria). To prepare substantial quantities of antibodies, scientists used to inject an antigen into an animal, wait for antibodies to form, draw blood from the animal, and isolate the antibodies. The antibodies obtained by this procedure were almost never pure, because typical antigens possess many recognizable surface sites, each of which leads to formation of a different type of antibody. Khler and Milstein saw that if a way could be found to clone lymphocytesto cause them to subdivide indefinitely in a culture mediumthen the antibody molecules secreted by the resulting population would all be identical. Lymphocytes are short-lived, however, and cannot be cultivated satisfactorily. Khler and Milstein solved this problem by inducing lymphocytes to fuse with the cells of a myeloma (a type of tumour), which can be made to reproduce indefinitely. The resulting hybrid cells produced a single species of antibody while perpetuating themselves indefinitely. The development of monoclonal antibodies revolutionized many diagnostic procedures and led to new therapeutic agents for fighting disease, since monoclonal antibodies can be designed to target specific types of cells or other antigens and can be used to carry drugs to those cells. Khler worked at the Basel Institute for Immunology from 1976 to 1985. In 1985 he was appointed one of three directors of the Max Planck Institute for Immunobiology in Freiburg.